Direct organogenesis from hypocotyl explants of <i style="">Psoralea corylifolia </i>L.—An endangered medicinal plant

Abstract

A protocol has been developed for <i style="">in vitro </i>regeneration of <i style="">Psoralea corylifolia </i>L. through adventitious shoot regeneration from hypocotyl explants cultured on Murashige and Skoog (MS) medium supplemented with various combinations and concentrations of plant growth regulators. The highest response was obtained on MS medium containing 3.0 µ<i style="">M</i> benzyladenine (BA), 1.0 µ<i style="">M</i> naphthalene acetic acid (NAA), 5.0 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> ascorbic acid (AA) and 100 mg L^-1casein hydrolysate (CH). The regenerated shoots were rooted on half-strength MS basal medium supplemented with 3.0 µ<i style="">M</i> indole-3-butyric acid (IBA). Rooted shoots were effectively transferred to garden soil, farmyard soil and sand (2:1:1 ratio) mixture. Subsequently, the plants were successfully acclimatized. During acclimatization, the plants were irrigated with 50 mL one-eighth strength MS basal salt solution devoid of sucrose and inositol, but supplemented with 3.0 <img src='/image/spc_char/micro.gif' border=0> <i style="">M</i> IBA and 100 mg L^–1 bavistin (BVN). The plants were subsequently established in the field.