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Title: In vitro multiplication of Asteracantha longifolia (L.) Nees–A medicinal herb
Authors: Panigrahi, J
Mishra, R R
Behera, M
Keywords: Asteracantha longifolia;leaf explant;organogenesis;shoot bud
Issue Date: Oct-2006
Publisher: CSIR
IPC Code: Int. Cl.8 A01H4/00
Abstract: Plantlet regeneration in Asteracantha longifolia (L.) Nees (Acanthaceae), a medicinal herb, has been achieved from leaf segments of seedlings germinated on basal MS medium. Leaf explants were cultured on MS medium fortified with various concentrations of BA and NAA either alone or in combination. Among the combinations used, BA(2.0 mg/L) + NAA (0.2 mg/L) was found to be the best for direct multiple shoot bud induction, while BA(2.0 mg/L) + NAA(0.5 mg/L) was suitable for high frequency shoot bud regeneration through an intervening callus phase. Well-developed shoots (>3 cm) were successfully rooted on MS medium containing NAA (0.1 mg/L). Eighty percent of the regenerated plantlets were successfully established in soil. The protocol described is simple, rapid, and efficient for in-vitro propagation of A. longifolia from leaf explants and soil establishment of plants.
Page(s): 562-564
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.05(4) [October 2006]

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