Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/7751| Title: | Occurrence of Iris mild mosaic potyvirus in cultivated iris in India |
| Authors: | Kulshrestha, Saurabh Hallan, Vipin Raikhy, Gaurav Ram, Raja Garg, I D Haq, Q M R Zaidi, A A |
| Keywords: | cytopathology;ELISA;IC-RT-PCR;IEM;Iris mild mosaic potyvirus (IMMV) |
| Issue Date: | Jan-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 C07K16/10; C12N15/10; G01N33/53 |
| Abstract: | Iris (Iris x hollandica Hort. cv. Bluemagic) plants showing mosaic symptoms were tested for the presence of Iris mild mosaic potyvirus (IMMV) by host range, enzyme linked immunosorbent assay (ELISA) using antibodies specific for IMMV), reverse transcription polymerase chain reaction (RT-PCR), immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) using potyvirus group specific primers and antibodies specific to IMMV, immune electron microscopy and cytopathology. Presence of IMMV was detected by ELISA, confirmed by RT-PCR, IC-RT-PCR, IEM, cytopathology and sequencing of the cloned PCR product. From the sequence of the virus, specific primers were designed and specific detection of IMMV from iris was standardized. |
| Page(s): | 94-98 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.05(1) [January 2006] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 5(1) 94-98.pdf | 452.23 kB | Adobe PDF | View/Open |
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.