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Title: In vitro propagation and microrhizome induction in Kaempferia galanga Linn. and K. rotunda Linn.
Authors: Chirangini, P
Sinha, S K
Sharma, G J
Keywords: 6-benzylaminopurine (BAP);indole-3-acetic acid (IAA);Kaempferia, kinetin (Kn);microrhizome; -naphthalene acetic acid (NAA)
Issue Date: Jul-2005
Publisher: CSIR
IPC Code: Int. Cl.7 A01H4/00, 5/04
Abstract: Rhizomatous buds of Kaempferia galanga and K. rotunda induced microshoots when cultured on Murashige and Skoog (MS) medium supplemented with plant growth regulators. Multiple shoots were induced on MS medium containing 5.70 M IAA alone and a combination of 0.57 M IAA plus 4.65 M Kn in case of K. galanga. Whereas, the medium supplemented with 2.69 M NAA plus 2.22 M BAP was the best for K. rotunda. Further, subculture of the microshoots gave more multiple shoots (13) on medium containing 4.44 M BAP in K. galanga and (9) on the 2.69 M NAA and 2.22 M BAP in K. rotunda. Induction of callus was observed in K. galanga on the medium supplemented with 2.85 M IAA. Microshoots were regenerated from the callus on 2.69 M NAA and 2.22 M BAP enriched medium. Microrhizome formation was observed within one month of incubation of microshoot cultures (~4 month old, following 4 passages) in the medium supplemented with 6-9% sucrose with either 22.2 M BAP or 23.25 M Kn. These microrhizomes produced shoots when transferred to fresh microshoot induction medium within 2-3 weeks of incubation. The microshoots produced roots irrespective of their method of regeneration. Plantlets transplanted to pots grew to mature plants after 3 months of transfer and showed 80-90% survival.
Page(s): 404-408
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.04(3) [July 2005]

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