<i style="">In vitro </i>regeneration and mass multiplication of <i style="">Psoralea corylifolia</i>—An endangered medicinal plant

Abstract

An efficient protocol has been developed for plant regeneration from shoot tip and nodal explants of <i style="">in vitro </i>grown <i style="">Psoralea corylifolia</i>. Nodal segments were more morphogenic to shoot bud differentiation than shoot tips. Proliferation of shoots was achieved on Murashige and Skoog (MS) medium supplemented with various concentrations of BA, Kn, IAA, NAA either singly or in various combinations. The highest shoot regeneration frequency (90%) and number of shoots (12.0 ± 0.57) were obtained from nodal segment on MS medium fortified with 5 µ<i style="">M</i> BA and 0.5 µ<i style="">M</i> NAA. Addition of CH (100 mg/l) to the shoot induction medium enhanced the growth of regenerants. The regenerated shoots rooted best on MS medium containing 0.5 µ<i style="">M</i> IBA. Regenerated plantlets with well-developed shoot and roots were hardened, successfully transferred to soil and maintained in green house. The present <i style="">in vitro</i> procedure can be used in conservation and mass propagation of this endangered medicinal plant.