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Title: A study on accumulation of PHB in native Pseudomonas isolates LDC-5 and LDC-25
Authors: Sujatha, K
Mahalakshmi, A
Shenbagarathai, R
Keywords: polyhydroxyalkanoate;PHA synthase;colony PCR;seminested PCR;biodegradable plastics;Pseudomonas
Issue Date: Apr-2005
Publisher: CSIR
IPC Code: Int. Cl.7 C08G63/00; C12N15/10; C12R1:38
Abstract:  Poly (-hydroxyalkanoates) (PHAs) are natural polyesters produced by a variety of bacteria.They are represented most commonly by poly (-hydroxybutyrate) (PHB), an intracellular storage biodegradable polymer material. The production costs of PHB are quite high compared with those of synthetic non-degradable plastics, hence search for potential strains with high PHB accumulating ability. Hundreds of indigenous bacterial strains were screened for the accumulation of PHB by Nile red, fluorescence microscopy (Nile blue A) and PCR. Three degenerate primers were used as PCR primers to detect PHA synthase genes. Among the tested isolates, 35 strains yielded a specific amplicon of 496 bp and 406 bp in colony PCR and seminested PCR, respectively. Among the 35 short chain length positive strains, only 2 isolates yielded a specific amplicon of 540 bp PCR product in medium chain length PCR, representing partial coding sequences of phaC1/phaC2 genes. The mcl-PCR positive Pseudomonas indigenous isolates (LDC-5 and LDC-25) could be potential candidates for bioplastic production.
Page(s): 216-221
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.04(2) [April 2005]

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