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http://nopr.niscpr.res.in/handle/123456789/5956| Title: | Construction of dissolved O2 metric oxalate biosensor using PVC membrane bound sorghum leaf oxalate oxidase |
| Authors: | Pundir, C S Phaugat, Neelam |
| Keywords: | Dissolved oxygen meter;Oxalate biosensor;PVC membrane;Sorghum leaf oxalate oxidase |
| Issue Date: | Sep-2009 |
| Publisher: | CSIR |
| Abstract: | A portable dissolved oxygen (DO) meter based oxalate biosensor has been constructed by mounting a polyvinyl chloride (PVC) membrane bound sorghum leaf oxalate oxidase on sensing part of combined electrode of an aqualytic DO meter. Biosensor showed optimum activity within 10-12 s at pH 5.0 and 30°C. A linear relationship was observed between oxalate conc. and O2 consumption [1.0-10 μM (low conc. range), 0.1-1.0 mM (high conc. range)]. Biosensor gave mean value of oxalic acid in urine [22.5 mg/l in males (n=6) and 19.3 mg/l in females (n=6)], and in serum [3.2μM/l in males (n=6) and 2.9 μM/l in females (n=6)]. Biosensor is unaffected by physiological concentrations of Cl- and NO3-. |
| Page(s): | 800-806 |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Appears in Collections: | JSIR Vol.68(09) [September 2009] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| JSIR 68(9) 800-806.pdf | 197.41 kB | Adobe PDF | View/Open |
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