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Title: Silver nitrate induced rooting and flowering in vitro on rare rhoeophytic woody medicinal plant, Rotula aquatica Lour.
Authors: Chithra, M
Martin, K P
Sunandakumari, C
Madhusoodanan, P V
Keywords: Rotula aquatica;liquid medium;plantlet survival;solid medium
Issue Date: Jul-2004
Publisher: CSIR
IPC Code: Int. Cl.7 A 01 H 4/00, 5/02, 5/06
Abstract: Silver nitrate induced enhanced rooting and flowering in vitro was achieved on Rotula aquatica L., a rare rhoeophytic woody aromatic medicinal plant. Solid and liquid nature of the medium, and growth regulators significantly influenced in vitro rooting and flowering. Half-strength MS liquid medium fortified with 2.69 M -naphthaleneacetic acid (NAA) was effective among different auxins, which induced a mean of 7.3 roots per shoot. Plantlets acclimatized after auxin alone induced rooting exhibited 80% survival in field conditions. Nevertheless, the established plantlets exhibited 50% loss within 3 months. Addition of 11.7 M silver nitrate to half-strength MS liquid medium containing 2.69 M NAA increased the number of roots to 16.8 per shoot. Plantlets established after rooting in silver nitrate supplemented medium facilitated 95% survival, and the established plantlets did not exhibit loss even after 3 months. Dipping of the basal end of shoots developed in vitro in NAA (2.69 M) and silver nitrate (11.7 M) solution (made in water) for 25 days induced a mean of 12.4 roots per shoot. These plantlets transferred to small pots facilitated 90% survival. Half-strength MS liquid medium containing 2.69 M NAA and 11.7 M silver nitrate induced flowering earlier. The flowers were morphologically and structurally similar to that on plants growing in field conditions.
Page(s): 418-421
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(3) [July 2004]

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