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Title: Somatic embryogenesis and plantlet regeneration from cotyledon and leaf explants of Solanum surattense
Authors: Swamy, N Rama
Ugandhar, T
Praveen, M
Venkataiah, P
Rambabu, M
Upender, M
Subhash, K
Keywords: S. surattense;Indian solanum;somatic embryos;regeneration
Issue Date: Jul-2005
Publisher: CSIR
IPC Code: Int. Cl.7 A01H 4/00, 5/10, 5/12
Abstract: Somatic embryogenesis and plant regeneration from the cotyledon and leaf explants of Indian Solanum (Solanum surattense Burm. f.), a medicinally important plant, is reported. Embryogenic callus was induced from cotyledon and leaf explants on Murashige and Skoog’s medium fortified with 0.5-8.0 mg/L -naphthalene acetic acid (NAA) + 0.5 mg/L N6-benzylaminopurine (BAP). High frequency of somatic embryo formation was found at 6.0 mg/L NAA + 0.5 mg/L BAP and 4.0 mg/L NAA+ 0.5 mg/L BAP in cotyledon and leaf explants, respectively. Secondary somatic embryogenesis was also observed when primary somatic embryos were subcultured on the same somatic embryo induction medium. Well-developed cotyledonary stage embryos were germinated on MS medium supplemented with 0.5 mg/L indole-3-acetic acid (IAA) +1.0-8.0 mg/L BAP. Maximum percentage (71.2%) of somatic embryo germination and plantlet formation was found at 0.5 mg/L IAA+2.0 mg/L BAP, but they didn’t germinate on ½ MSO and MSO media. The post transplantation survival rate of plants was 65-70%. Plants and flowers formed were morphologically similar to mother plants. The present protocol can be used for genetic transformation experiments in S. surattense.
Page(s): 414-418
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.04(3) [July 2005]

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