Analysis of VNTR loci, ApoB 3' HVR and D1S80 in North Indians

Abstract

Polymorphic markers like VNTRs at Apolipoprotein B 3' and locus D1S80 hypervariable region have been used extensively for population studies through out the world. In the present study, the polymorphism data in North Indian population at these VNTR loci was reported. The allele distributions and their genotype frequencies at the VNTR loci, Apo B and D1S80 were reported in 86 (172 chromosomes) and 75 (150 chromosomes) unrelated normal individuals, respectively. Genomic DNA was extracted from blood samples and amplified by polymerase chain reaction. The respective alleles and their sizes were determined, 19 and 24 different alleles making up 51 and 50 genotypes of Apo B and D1S80 respectively were identified in the North Indian population. As per <img src='/image/spc_char/lembda.gif'>² analysis, the allele and genotype frequencies for both VNTRs were in Hardy-Weinberg equilibrium. The most frequent allele of Apo B (allele 6) corresponded to 40 repeats, and D1S80 (allele 12) to 28 repeats. The frequencies were 0.087 and 0.17 and observed heterozygosities were 55 and 57% for Apo B and D1S80, respectively. This information may have implications in disease diagnostics, forensics, paternity analysis, and for ruling out maternal contamination in fetal samples during prenatal diagnosis of genetic disorders.