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Title: Degradation of phorate by Azotobacter isolates
Authors: Kadam, T A
Gangawane, L V
Keywords: Azotobacter;N fixation;phorate;tolerance
Issue Date: Jan-2005
Publisher: CSIR
IPC Code: Int. Cl.7 A 01 N 63/02
Abstract:  Of 44 Azotobacter isolates from the soils of Maharashtra, 29 belonged to A. chroococcum and other were A. vinelandii. All A. chroococcum isolates were efficient in N fixation when tested by acetylene reduction test and also in cotton cv NHH-44. However, they showed variation to phorate tolerance. The maximum tolerance (150 g/ml) was shown by ACI-17, ACI-18 and ACI-28 isolates. These isolates also showed maximum phorate degradation. TLC and HPLC studies indicated the presence of O,O-diethyl S-{(ethyl sulphonyl) methyl} phosphorothioate, O,O-diethyl S-{(ethyl sulphinyl) methyl} phosphorothioate and diethyl phosphoric acid as degradation products. Plasmid analysis indicated that the genes for phorate degradation might be located on the main chromosome and not on the plasmid.
Page(s): 153-155
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.04(1) [January 2005]

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