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DC Field | Value | Language |
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dc.contributor.author | Kumar, Devendra | - |
dc.contributor.author | Nehra, Priyanka | - |
dc.contributor.author | Saxena, Sudhir | - |
dc.contributor.author | Dhar, Dolly Wattal | - |
dc.date.accessioned | 2019-08-01T04:48:02Z | - |
dc.date.available | 2019-08-01T04:48:02Z | - |
dc.date.issued | 2019-08 | - |
dc.identifier.issn | 0975-1009 (Online); 0019-5189 (Print) | - |
dc.identifier.uri | http://nopr.niscair.res.in/handle/123456789/49541 | - |
dc.description | 625-629 | en_US |
dc.description.abstract | The cyanobacteria Anabaena and Phormidium are potential source of phycobiliproteins and C phycocyanin (C-pc). Here, we carried out extraction and purification of phycocyanin (PC) from the above selected cyanobacterial isolates using one-step anion exchange chromatography. Crude C-phycocyanins were extracted and concentrated by ammonium sulfate fractionation at saturation of 35%, then purified on a DEAE-sepharose with Fast Flow chromatography column having continuous pH gradient elution from pH 5.1 to 3.76. The process resulted in recovery of high purity C-pc from above cyanobacteria. The purity ratios (A620/A280) of phycocyanin reached 3.34 for Phormidium and 3.1 for Anabaena, respectively. The purity was further demonstrated and confirmed through fluorescence emission spectroscopy. The total recovery yield of pure C-pc was 14% after completion of the process, and the recovered pigment remained stable over a pH range of 4-9. This purification method for recovery of high purity pigment was fairly efficient compared to the existing methods. . As phycocyanin has higher antioxidant activity and hence, the above cyanobacterial strains Anabaena and Phormidium with considerable amount of C-pc, may serve to be a potential source as food supplement as well as for pharmaceuticals industries. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | NISCAIR-CSIR, India | en_US |
dc.rights | ![]() | en_US |
dc.source | IJEB Vol.57(08) [August 2019] | en_US |
dc.subject | Anion Exchange Chromatography | en_US |
dc.subject | DEAE Cellulose | en_US |
dc.subject | Phycobiliproteins | en_US |
dc.title | Quantification and purification of C-phycocyanin from cyanobacterial strains Anabaena and Phormidium | en_US |
dc.type | Article | en_US |
Appears in Collections: | IJEB Vol.57(08) [August 2019] |
Files in This Item:
File | Description | Size | Format | |
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IJEB 57(8) 625-629.pdf | 147.97 kB | Adobe PDF | View/Open |
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