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Title: Arsenite-oxidation performance of microbes from abandoned iron ore mine
Authors: Thul, Sanjog T
Nigam, Bhavna
Tiwari, Sarita
Pandey, Ram A
Keywords: Arsenopyrite;Arsenic transformation;Silver nitrate test;HPLC-ICP-MS
Issue Date: Jan-2019
Publisher: NISCAIR-CSIR, India
Abstract: Arsenite is considered to be more toxic than arsenate (an average of 100 times). It can be oxidized to arsenate by chemical reaction or microbiological interactions. The aim of the present study was to investigate the arsenite-oxidizing microbes that exist in iron ore mine in India. We cultured 13 morphologically distinct bacterial strains, among which 6 strains could grow in high concentrations and shows the arsenite transforming abilities. Analysis of the amplified 16S rDNA gene sequences of the isolates revealed them to belong to alpha-, gamma- proteobacteria and firmicutes particularly in genera Paenibacillus, Pseudomonas, Ochrobactrum, Enterobacter and Bacillus. Further, qualitative silver nitrate screening assay and quantification by HPLC-ICP-MS analysis of these strains indicated the transformation of arsenite to arsenate. Moreover, the isolates were genetically analyzed for presence of arsenic arsenite transporter gene (arsB) that indicates the genetic ability of bacteria to tolerate the most toxic arsenic species.
Page(s): 34-41
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.18(1) [January 2019]

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