Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/4641| Title: | Cost effectiveness of colony lysis and colony PCR methods for screening of recombinant Escherichia coli colonies ¾ A comparative study |
| Authors: | Azhahianambi, P Ghosh, S Kumar, C Ashok Suryanarayana, V V S |
| Keywords: | Colony lysis;Colony PCR;Cost effectiveness;Recombinant E. coli |
| Issue Date: | Oct-2008 |
| Publisher: | CSIR |
| Abstract: | Economizing the research protocols by using low cost technologies is the need of laboratories of developing world. Screening of recombinant E. coli colonies is the crucial step in gene cloning and expression studies. In the present study, the cost effectiveness of colony lysis method and colony PCR method in the screening of recombinant E. coli colonies was compared. The colony lysis method was 20 two times more cost effective and less time consuming and can be used to screen the recombinant E. coli colonies in large scale instead of colony PCR method. |
| Page(s): | 731-735 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.46(10) [October 2008] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 46(10) 731-735.pdf | 301.61 kB | Adobe PDF | View/Open |
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