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DC Field | Value | Language |
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dc.contributor.author | Swetha, V P | - |
dc.contributor.author | Parvathy, V A | - |
dc.contributor.author | Sheeja, T E | - |
dc.contributor.author | Sasikumar, B | - |
dc.date.accessioned | 2018-10-23T11:52:02Z | - |
dc.date.available | 2018-10-23T11:52:02Z | - |
dc.date.issued | 2018-07 | - |
dc.identifier.issn | 0975-0967 (Online); 0972-5849 (Print) | - |
dc.identifier.uri | http://nopr.niscair.res.in/handle/123456789/45270 | - |
dc.description | 533-537 | en_US |
dc.description.abstract | A reliable and efficient protocol for isolation and amplification of genomic DNA from dried mace of Myristica fragrans, was developed. The yield of genomic DNA was 231.4 µg g-1 and 306.8 µg g-1, respectively for the samples procured from the farm and market. The absorbance ratio at A260/A280 was greater than 1.8 indicating the good quality of DNA. Complete restriction digestion and PCR amplification of genomic DNA further confirmed the quality of isolated DNA. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | NISCAIR-CSIR, India | en_US |
dc.rights | CC Attribution-Noncommercial-No Derivative Works 2.5 India | en_US |
dc.source | IJBT Vol.17(3) [July 2018] | en_US |
dc.subject | Nutmeg | en_US |
dc.subject | Myristica fragrans | en_US |
dc.subject | Recalcitrant | en_US |
dc.subject | Restriction digestion | en_US |
dc.subject | RAPD | en_US |
dc.subject | rbcL | en_US |
dc.subject | ITS | en_US |
dc.title | Isolation and amplification of genomic DNA from nutmeg mace | en_US |
dc.type | Article | en_US |
Appears in Collections: | IJBT Vol.17(3) [July 2018] |
Files in This Item:
File | Description | Size | Format | |
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IJBT 17(3) 533-537.pdf | 575.76 kB | Adobe PDF | View/Open |
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