Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/45270| Title: | Isolation and amplification of genomic DNA from nutmeg mace |
| Authors: | Swetha, V P Parvathy, V A Sheeja, T E Sasikumar, B |
| Keywords: | Nutmeg;Myristica fragrans;Recalcitrant;Restriction digestion;RAPD;rbcL;ITS |
| Issue Date: | Jul-2018 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A reliable and efficient protocol for isolation and amplification of genomic DNA from dried mace of Myristica fragrans, was developed. The yield of genomic DNA was 231.4 µg g-1 and 306.8 µg g-1, respectively for the samples procured from the farm and market. The absorbance ratio at A260/A280 was greater than 1.8 indicating the good quality of DNA. Complete restriction digestion and PCR amplification of genomic DNA further confirmed the quality of isolated DNA. |
| Page(s): | 533-537 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.17(3) [July 2018] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 17(3) 533-537.pdf | 575.76 kB | Adobe PDF | View/Open |
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