Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/45090| Title: | A two locus barcode for discriminating Piper nigrum from its related adulterant species |
| Authors: | Parvathy, V A Swetha, V P Sheeja, T E Sasikumar, B |
| Keywords: | DNA barcoding;psbA-trnH;rpoC1;rbcL. Piper nigrum;Single locus |
| Issue Date: | Apr-2018 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | DNA barcoding in plants involves the selection and sequencing of DNA regions as a tool for species identification. Here we have attempted to discriminate Piper nigrum L., black pepper of commerce, from its related adulterant species viz., Piper attenuatum Buch-Ham, Piper galeatum (Miq.) C. DC. using rbcL, psbA-trnH, rpo'C'l, and matK as barcode loci. No single barcoding locus could discriminate all the three species of Piper. However rbcL and rpoC1 could differentiate P. attenuatum from P. nigrum and Piper galeatum while psbA-trnH differentiated P. galeatum from P. nigrum and P. attenuatum. Two locus barcode approach proved better when compared to single locus in discriminating Piper species. The coding rbcL gene with non coding psbA-trnH spacer was preferred than the coding rpoC1 + psbA-trnH combination in the present study. |
| Page(s): | 346-350 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.17(2) [April 2018] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 17(2) 346-350.pdf | 1.08 MB | Adobe PDF | View/Open |
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.