Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSaxena, J-
dc.contributor.authorChoudhary, N-
dc.contributor.authorGupta, P-
dc.contributor.authorSharma, M M-
dc.contributor.authorSingh, A-
dc.identifier.issn0975-1084 (Online); 0022-4456 (Print)-
dc.description.abstractProteases account for nearly 60% of the industrial enzyme market and have wide applications. Present study has been designed to isolate and screen protease producing fungi from various soil samples of Jaipur region and optimum physico-cultural conditions for enhanced protease production from selected isolate was also investigated. Isolate fungi were screened by growing them onto skim milk agar plates and the zone of proteolysis was noted. Isolate FGCC/BLS2 showed maximum hydrolysis capacity when compared to wild type positive reference strain Aspergillus flavus. Molecular Characterization of the FGCC/BLS 2 isolate confirmed it as Cladosporium sp. PAB 2014 strain FGCC/BLS 2 (Submitted to Gen Bank: Accession Number KU 752193). The highest enzyme activity was obtained with production media at 120 hour (60.9±2.17 U/mg protein) with 2% inoculums (63.19±0.59 U/mg protein), pH 7 (63.77±2.45 U/mg protein), dextrose as the carbon source (63.9±1.63 U/mg protein) and tryptone as a nitrogen source (66.9±2.34 U/mg protein). The optimum conditions for protease assay was found to be 40oC temperature, 1.5% substrate concentration and at pH 7.0 respectively.en_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceJSIR Vol.76(11) [November 2017]en_US
dc.subjectCladosporium spen_US
dc.subjectSubmerged Conditionen_US
dc.titleIsolation and Characterization of Neutral Proteases Producing Soil fungus Cladosporium sp PAB2014 Strain FGCC/BLS2: Process Optimization for Improved Enzyme Productionen_US
Appears in Collections:JSIR Vol.76(11) [November 2017]

Files in This Item:
File Description SizeFormat 
JSIR 76(11) 707-713.pdf387.11 kBAdobe PDFView/Open

Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.