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Title: Evaluation of genetic diversity using random amplified polymorphic DNA (RAPD) markers in Melia dubia Cav.
Authors: Johar, Vishal
Kajla, Subhash
Dhillon, R S
Bhatia, Pankaj
Keywords: Candidate plus trees;Genetic diversity;Melia dubia;M. composita;Polymorphism;RAPD;UPGMA
Issue Date: Jan-2017
Publisher: NISCAIR-CSIR, India
Abstract: Random amplified polymorphic DNA (RAPD) molecular markers were used to evaluate the genetic diversity in populations of Melia dubia Cav. syn. M. composita Willd. (Family: Meliaceae) (Burma dek) from different agroclimatic regions of India. Of the 38 decamer primers used, 13 yielded polymorphic banding patterns. In total, 105 different bands were reproducibly obtained, of which 69 (65.7%) were polymorphic. The polymorphisms were scored and used in band-sharing analysis to identify genetic relationships. Cluster analysis based on Jaccard’s similarity coefficient using UPGMA grouped all the 24 populations into two major groups. Similarity indices ranged from 0.80 to 0.91, indicating that Burma dek germplasm within India constitutes considerably narrow genetic base.
Page(s): 76-83
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.16(1) [January 2017]

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