Effect of Tocotrienol on medium dose ethanol-induced alternations in serum superoxide and peroxide handling capacities (SPHC) in rat

Abstract

Tocotrienols are members of vitamin E family present in low concentrations and possess high antioxidant activity. Consumption of ethanol is a common problem and induces oxidative stress. In this study, we evaluated the effect of tocotrienol against ethanol-induced oxidative stress. Male albino Wistar rats were divided into two sets; one set of rats were exposed with low to moderate doses of ethanol for 4 weeks, while another set was exposed to tocotrienol orally (10 mg/day) in addition to the ‘low to moderate doses of ethanol for 4 weeks’. Oxidative stress parameters, like levels of reduced glutathione and lipid peroxidation, activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase were determined in serum before the initiation of treatment protocol and at the end of 2nd and 4th week of treatment. Serum levels of superoxide and peroxide handling capacities were also calculated in those three time points. Tocotrienol-treated rats showed statistically significant enhancement in reduced glutathione level, glutathione peroxidase and glutathione reductase activities. Glutathione-dependent superoxide and peroxide handling capacity of those rats were found to be higher. The current study suggests that the tocotrienol-induced protection against the oxidative stress is most likely mediated by glutathione-based system.