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http://nopr.niscpr.res.in/handle/123456789/34069| Title: | Scavenging and antioxidant properties of different grape cultivars against ionizing radiation-induced liver damage ex vivo |
| Authors: | Singha, Indrani Das, Subir Kumar |
| Keywords: | Gamma ( ) radiation;ROS;Vitis vinifera |
| Issue Date: | Apr-2016 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Ionizing radiation (IR) has become an integral part
of the modern medicine— both for diagnosis as well as therapy. However, normal
tissues or even distant cells also suffer IR-induced free radical insult. It
may be more damaging in longer term than direct radiation exposure. Antioxidants
provide protection against IR-induced damage. Grapes
are the richest source of antioxidants. Here, we
assessed the scavenging properties of four grape (Vitis vinifera)
cultivars, namely Flame seedless (Black), Kishmish chorni (Black with reddish
brown), Red globe (Red) and Thompson seedless mutant (Green), and also
evaluated their protective action against
-radiation-induced
oxidative stress in liver tissue ex vivo. The scavenging abilities of
grape seeds [2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50=0.008±0.001
mg/mL), hydrogen peroxide (IC50=0.49 to 0.8 mg/mL), hydroxyl
radicals (IC50=0.08±0.008 mg/mL), and nitric oxide (IC50=0.8±0.08
mg/mL)] were higher than that of skin or pulp. Gamma () radiation exposure to sliced liver tissues ex vivo from goat, @ 6 Gy significantly
(P <0.001) decreased reduced glutathione (GSH) content by 21.2% and
also activities of catalase, glutathione peroxidase (GPx), glutathione
reductase (GR) and glutathione s-transferase (GST) by 49.5, 66.0, 70.3, 73.6%,
respectively. However, it increased thiobarbituric acid reactive substances
(TBARS) by 2.04-fold and nitric oxide level by 48.6% compared
to untreated group. Further increase in doses (10 or 16 Gy) of -radiation
correspondingly decreased GSH content and enzyme activities, and increased
TBARS and nitric oxide levels. Grape extract treatment prior to ionizing
radiation exposure ameliorated theses effects at varying extent. The seed
extracts exhibited strong antioxidant potential compared to skin or pulp
extracts of different grape cultivars against oxidative damage by ionizing
radiation (6 Gy, 10 Gy and 16 Gy) in sliced liver tissues
ex vivo. Grape extracts at
higher concentration (10 mg extract/g liver tissue)
showed stronger antioxidant potential against lower dose
(6 Gy) of ionizing radiation. Our results suggest that grape
extracts could serve as a potential source of natural antioxidant against lower
doses of IR-induced oxidative stress in liver extracts ex vivo. |
| Page(s): | 280-285 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.54(04) [April 2016] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 54(4) 280-285.pdf | 422.03 kB | Adobe PDF | View/Open |
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