Please use this identifier to cite or link to this item:
Title: mRNA and Protein levels of rat pancreas specific protein disulphide isomerase are downregulated during Hyperglycemia
Authors: Gupta, Rajani
Bhar, Kaushik
Sen, Nandini
Bhowmick, Debajit
Mukhopadhyay, Satinath
Panda, Koustubh
Siddhanta, Anirban
Keywords: Beta cell;Diabetes;Insulin;Protein disulphide isomerase (Pdi)
Issue Date: Feb-2016
Publisher: NISCAIR-CSIR, India
Abstract: Diabetes (Type I and Type II) which affects nearly every organ in the body is a multi-factorial non-communicable disorder. Hyperglycemia is the most characteristic feature of this disease. Loss of beta cells is common in both types of diabetes whose detailed cellular and molecular mechanisms are yet to be elucidated. As this disease is complex, identification of specific biomarkers for its early detection, management and devising new therapies is challenging. Based on the fact that functionally defective proteins provide the biochemical basis for many diseases, in this study, we tried to identify differentially expressed proteins during hyperglycemia. For that, hyperglycemia was induced in overnight fasted rats by intra-peritoneal injection of streptozotocin (STZ). The pancreas was isolated from control and treated rats for subsequent analyses. The 2D-gel electrophoresis followed by MALDI-TOF-MS-MS analyses revealed several up- and down- regulated proteins in hyperglycemic rat pancreas including the downregulation of a pancreas specific isoform of protein disulphide isomerase a2 (Pdia2).This observation was validated by western blot. Quantitative PCR experiments showed that the level of Pdia2 mRNA is also proportionally reduced in hyperglycemic pancreas.
Page(s): 100-107
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.54(02) [February 2016]

Files in This Item:
File Description SizeFormat 
IJEB 54(2) 100-107.pdf371.72 kBAdobe PDFView/Open

Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.