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Title: Isolation and quantification of six cardiac glycosides from the seeds of Thevetia peruviana provide a basis for toxological survey
Authors: Kohls, Sarah
Scholz-Böttcher, Barbara M
Teske, Jörg
Rullkötter, Jürgen
Keywords: Accelerated solvent extraction;LC-ESI+-MS/MS;Thevetia peruviana seeds;Quantification;Yellow Oleander
Issue Date: Dec-2015
Publisher: NISCAIR-CSIR, India
Abstract: In order to support toxicological assessment of poisoning from the ingestion of plant material containing thevetia cardiac glycosides, a new isolation method has been developed for thevetia glycosides from the seeds of the Yellow Oleander [Thevetia peruviana (Pers.) K. Shum, Apocynaceae] with yellow and orange flowers, respectively, using accelerated solvent extraction. The relative proportions of four cardiac-active thevetia glycosides (thevetin A and B, acetylthevetin A and B) and two additional, cardiac-inactive thevetia glycosides (thevetin C and acetylthevetin C) in the extract were determined using an LC-ESI+-MS/MS technique. Absolute quantification of thevetin B was achieved using a pure standard of this compound isolated by preparative high performance liquid chromatography.
Page(s): 1502-1510
ISSN: 0975-0983(Online); 0376-4699(Print)
Appears in Collections:IJC-B Vol.54B(12) December 2015]

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