Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/33513| Title: | Isolation and quantification of six cardiac glycosides from the seeds of Thevetia peruviana provide a basis for toxological survey |
| Authors: | Kohls, Sarah Scholz-Böttcher, Barbara M Teske, Jörg Rullkötter, Jürgen |
| Keywords: | Accelerated solvent extraction;LC-ESI+-MS/MS;Thevetia peruviana seeds;Quantification;Yellow Oleander |
| Issue Date: | Dec-2015 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In order to support toxicological assessment of poisoning from the ingestion of plant material containing thevetia cardiac glycosides, a new isolation method has been developed for thevetia glycosides from the seeds of the Yellow Oleander [Thevetia peruviana (Pers.) K. Shum, Apocynaceae] with yellow and orange flowers, respectively, using accelerated solvent extraction. The relative proportions of four cardiac-active thevetia glycosides (thevetin A and B, acetylthevetin A and B) and two additional, cardiac-inactive thevetia glycosides (thevetin C and acetylthevetin C) in the extract were determined using an LC-ESI+-MS/MS technique. Absolute quantification of thevetin B was achieved using a pure standard of this compound isolated by preparative high performance liquid chromatography. |
| Page(s): | 1502-1510 |
| ISSN: | 0975-0983(Online); 0376-4699(Print) |
| Appears in Collections: | IJC-B Vol.54B(12) December 2015] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJCB 54B(12) 1502-1510.pdf | 325.58 kB | Adobe PDF | View/Open |
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.