<span style="font-size:11.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-GB">Inhibition potential of <i style="mso-bidi-font-style:normal">Moringa oleifera</i> Lam. on drug metabolizing enzymes</span>

Abstract

<span style="font-size:11.0pt;font-family: " times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-in;mso-bidi-language:="" hi"="" lang="EN-GB">The leaves of <i style="mso-bidi-font-style:normal">Moringa oleifera</i> Lam. (Family:<span style="font-size:11.0pt;font-family:" times="" new="" roman","serif";="" mso-fareast-font-family:"times="" roman";mso-bidi-font-family:mangal;="" mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-GB"> Moringaceae)<span style="font-size:11.0pt;font-family:" times="" new="" roman","serif";="" mso-fareast-font-family:"times="" roman";mso-bidi-font-family:mangal;="" mso-ansi-language:en-gb;mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-GB"> has been traditionally used in Indian system of medicine (ISM) for its wide spectrum of pharmacological effects. The aim of this study was to evaluate the inhibitory effect of standardized <i style="mso-bidi-font-style:normal">M. oleifera</i> extract and its biomolecule chlorogenic acid on drug metabolizing enzymes (CYP450) which may cause interaction. The amount of chlorogenic acid was determined by using high performance thin layer chromatography (HPTLC). CYP-CO complex assay and fluorescence assay were followed for this study by using rat liver micro some (RLM) and two key isozymes CYP3A4 and CYP2D6. The hydro-alcoholic extract of <i style="mso-bidi-font-style:normal">M. oleifera</i> contains <span style="font-size:11.0pt;font-family:" times="" new="" roman","serif";="" mso-fareast-font-family:"times="" roman";mso-bidi-font-family:mangal;="" mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-GB">2.99 % <span style="font-size:11.0pt;font-family:" times="" new="" roman","serif";="" mso-fareast-font-family:"times="" roman";mso-bidi-font-family:mangal;="" mso-ansi-language:en-gb;mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-GB">(w/w) of chlorogenic acid by HPTLC analysis. The CYP-CO complex assay showed 23.45 <span style="font-size:11.0pt;font-family:" times="" new="" roman","serif";="" mso-fareast-font-family:"times="" roman";mso-bidi-font-family:mangal;="" mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-GB">± 0.78 <span style="font-size:11.0pt;font-family:" times="" new="" roman","serif";="" mso-fareast-font-family:"times="" roman";mso-bidi-font-family:mangal;="" mso-ansi-language:en-gb;mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-GB">% inhibition on RLM. The fluorescence study revealed that both the extract and chlorogenic acid (<span style="font-size:11.0pt;font-family: " times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">IC₅₀: 127.36 ± 2.98, 146.50 ± 3.46 µg/mL and 133.73 ± 1.79, 159.22 ± 3.53 µg/mL<span style="font-size:11.0pt;font-family: " times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-in;mso-bidi-language:="" hi"="" lang="EN-GB">) had some inhibition with both isozymes CYP3A4 and CYP2D6 but very less in comparison to their respective positive inhibitors ketoconazole and quinidine. Thus it may be concluded that <i style="mso-bidi-font-style:normal">M. oleifera</i> extract has very less potential to inhibit the CYP isozymes, so traditional use of this plant may be safe.</span></span></span></span></span></span></span></span></span>