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http://nopr.niscpr.res.in/handle/123456789/33015| Title: | Evaluation of Justicia adhatoda L. syn. Adhatoda vasica Nees extract by major metabolite analysis |
| Authors: | Sajeeb, B K Kumar, Uttom Rouf, A S S Uddin, Md Aftab Bachar, Sitesh C |
| Keywords: | Adhatoda vasica;Metabolite;Vasicine;Purification of vasicine;Quantitation of vasicine |
| Issue Date: | Oct-2015 |
| Publisher: | NISCAIR-CSIR, India |
| IPC Code: | Int. Cl.8: A61K 36/00, C01, C07, A61K 39/00, C12N |
| Abstract: | The present study reveals an improved method for the evaluation of Adhatoda vasica extract by quantitation of major metabolite vasicine. The plant powder was extracted with methanol and evaporated in vaccuo to obtain an oily residue. The residue was diluted with aqueous solution of citric acid (1%) and was further extracted with chloroform. The preliminary screening for alkaloid was done with alkaloidal reagents and the presence of vasicine was assessed by thin layer chromatography (TLC). The extractive was then subjected to column chromatography using a mixture of ethyl acetate and methanol adjusted to pH 9.5 with liquid ammonia as mobile phase. The TLC chromatograms of the collected column fractions were developed and the fractions indicated the presences of vasicine were combined together. The residue obtained from evaporation of solvent was subjected to preparative TLC along with reference standard to get pure crystalline vasicine. The compound vasicine was ascertained by UV-Vis, FT-IR and LCMS/MS analyses. The quantitative estimation of vasicine was carried out by reversed phase HPLC method. About 99.50% of pure vasicine (647 mg) compared to reference standard was obtained from 250 gm of leaf powder (0.2588% w/w). |
| Page(s): | 637-642 |
| ISSN: | 0975-1068 (Online); 0972-5938 (Print) |
| Appears in Collections: | IJTK Vol.14(4) [October 2015] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJTK 14(4) 637-642.pdf | 134.31 kB | Adobe PDF | View/Open |
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