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dc.contributor.authorKumar, Nagan Udhaya-
dc.contributor.authorGnanaraj, Muniraj-
dc.contributor.authorSindhujaa, Vajravel-
dc.contributor.authorViji, Maluventhen-
dc.contributor.authorManoharan, Kumariah-
dc.identifier.issn0975-1009 (Online); 0019-5189 (Print)-
dc.description.abstractA protocol for high frequency production of somatic embryos was worked out in pigeonpea, Cajanus cajan (L.) Millsp. The protocol involved sequential employment of embryogenic callus cultures, low density cell suspension cultures and a novel microdroplet cell culture system. The microdroplet cell cultures involved culture of a single cell in 10 µl of Murashige and Skoog’s medium supplemented with phytohormones, growth factors and phospholipid precursors. By employing the microdroplet cell cultures, single cells in isolation were grown into cell clones which developed somatic embryos. Further, 2,4-dichlorophenoxyacetic acid, kinetin, polyethylene glycol, putrescine, spermine, spermidine, choline chloride, ethanolamine and LiCl were supplemented to the low density cell suspension cultures and microdroplet cell cultures to screen for their cell division and somatic embryogenesis activity. Incubation of callus or the inoculum employed for low density cell suspension cultures and microdroplet cell cultures with polyethylene glycol was found critical for induction of somatic embryogenesis. Somatic embryogenesis at a frequency of 1.19, 3.16 and 6.51 per 106 cells was achieved in the callus, low density cell suspension cultures and microdroplet cell cultures, respectively. Advantages of employing microdroplet cell cultures for high frequency production of somatic embryos and its application in genetic transformation protocols are discussed.en_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJEB Vol.53(09) [September 2015]en_US
dc.subjectCallus cultureen_US
dc.subjectCholine chlorideen_US
dc.subjectCrop improvementen_US
dc.subjectLithium chlorideen_US
dc.subjectPlantlet regenerationen_US
dc.subjectPolyethylene glycolen_US
dc.titleA microdroplet cell culture based high frequency somatic embryogenesis system for pigeonpea, Cajanus cajan (L.) Millsp.en_US
Appears in Collections:IJEB Vol.53(09) [September 2015]

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