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Title: In vitro propagation of Spilanthes acmella (L.) Murray using semisolid and liquid medium
Authors: Joshi, Veenu
Tiwari, Kishan Lal
Jadhav, Shailesh Kumar
Keywords: Agar concentration;Antimalarial;Liquid medium;Propagation;Spilanthes acmella;Spilanthol
Issue Date: Jan-2015
Publisher: NISCAIR-CSIR, India
Abstract: Spilanthes acmella (L.) Murray (Family: Asteraceae) is a multifunctional medicinal plant, which is a major source of alkamides including spilanthol reported to exhibit various pharmacological activities. Therefore, an efficient in vitro propagation protocol is requisite for conserving and meeting the ever increasing industrial demands of this plant. The present study investigates the effect of agar concentration and liquid medium on in vitro proliferation of S. acmella. Nodal segments of a healthy plant were surface sterilized and inoculated on MS medium supplemented with different concentrations of BA (benzylaminopurine). Maximum bud break was observed in MS containing 0.5 mg/L BA. Maximum multiple shoots (6.20±0.45) with good mean shoot length (8.01±0.87 cm) was observed in liquid MS with 1 mg/L Kn (kinetin) in comparison with the semi solid medium. The regenerated shoots showed rooting without addition of any auxins but rooted best in MS medium containing 1 mg/L IBA and successfully hardened to ex vitro conditions using cocopeat irrigated with ½ MS, followed by transfer to soil with 100% survival.
Page(s): 112-116
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.14(1) [January 2015]

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