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Title: An improved method for Isolation of photosystem II from marine alga Porphyra yezoensis Udea
Authors: Gao, Z Q
Wang, G C
Tseng, C K
Keywords: Porphyra yeroensis Udea;Thylakoid membranes;PSII;Ultracentrifugation;Differential speeds centrifugation;SDS-PAGE;Absorption spectra;Fluorescence spectra
Issue Date: Feb-2005
Publisher: NISCAIR-CSIR, India
IPC Code: B01D 21/00
Abstract: An improved method for isolation and characterization of photosystem (PS)II particles from thylakoid membranes of gametophytes of a marine alga Porphyra yezoensis Udea is reported. Thylakoid membranes were isolated using ultracentrifugation and differential speeds centrifugation and were further purified by the first sucrose density gradient centrifugation (SOGC). PSII particles with high 2, 6- dichloroindophenol (OCIP) photo-reduction activity were isolated by the second SOGC from the thylakoid membranes. Absorption and fluorescence spectra of the thylakoid membranes and PSII particles were recorded and their polypeptides composition was studied. Thylakoid membranes obtained by the above two methods showed similar spectral properties and polypeptides composition. PSII particles, in addition to common extrinsic proteins found in PSII of other plants, contained cyt c-550, a 20 kDa protein, along with two newproteins (14 kDa and 16 kDa).
Page(s): 41-47
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.42(1) [February 2005]

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