Direct organogenesis and somatic embryogenesis in Beloperone plumbaginifolia (Jacq.) Nees

Abstract

Beloperone plumbaginifolia (Jacq.) Nees is a small branched medicinal shrub. In vitro studies were conducted, employing explants from node, internode, petiole, shoot bud and leaf lamina. Murashige and Skoog (MS) medium fortified with 6.66 μM BA enabled the proliferation of axillary and apical buds. MS medium supplemented with 5.37 μM NAA was better for callogenesis from nodal and internodal explants, while a combination of IBA (2.46 μM) and 2,4-D (4.52 μM) was good for leaf lamina explant. MS medium with 5.37 μM NAA and 2.22 μM BA was found superior for shoot induction from nodal explants. Half-strength MS medium with 5.37 μM NAA induced adventitious roots and 85% plantlets survived when transferred in the field conditions.