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Title: Callus induction and plant regeneration from leaf explants of jojoba [Simmondsia chinensis (Link) Schneider]
Authors: Kumar, Sunil
Mangal, Manisha
Dhawan, A K
Singh, Narender
Keywords: Callus;In vitro propagation;Jojoba;Simmondsia chinensis
Issue Date: Oct-2013
Publisher: NISCAIR-CSIR, India
Abstract: An efficient micropropagation protocol involving callus induction and shoot regeneration has been standardized in Simmondsia chinensis, an oil yielding, medicinal and multi-purpose plant species. Higher percent of callus proliferation (97.3%) was obtained from leaf explants, taken from field grown mature plant, when cultured on MS medium supplemented with 2,4-D (2.0 mg L-1)+BAP (0.5 mg L-1)+CH (100 mg L-1) within 20-22 d of inoculation. The callus was yellowish green in colour and soft in texture. Further, optimum shoot regeneration was obtained from the leaf derived callus on MS medium fortified with BAP (2.0 mg L-1)+NAA (0.5 mg L-1)+GA3 (0.3 mg L-1). About 92% cultures responded with an average number of 9.1 shoots per culture. The shoots obtained via callogenesis were rooted on half-strength agar-solidified MS medium supplemented with IBA (1.0 or 2.5 mg L-1). The medium containing 2.5 mg L-1 IBA was the best for rooting of shoots. The rooted shoots were transplanted to soil with 75% success. The protocol will be of immense importance in rapid mass multiplication of elite germplasm, as well as for conservation of this important species.
Page(s): 544-547
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.12(4) [October 2013]

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