Studies on biochemical and biological properties of turrids venom (<i>Turricula javana</i> <i>and</i> <i>Lophiotoma indica</i>) <b>[RETRACTED ARTICLE]</b>

Abstract

Turritoxins were isolated from the venom ducts of Turrid snails <i style="mso-bidi-font-style:normal">Turricula javana </i>and <i style="mso-bidi-font-style:normal">Lophiotoma indica</i> collected from Parangipettai waters, south east coast of India. Venom duct yield 1 g/Kg was dissected from both the species. Crude venom exhibited protein concentration of 0.138mg/ml and 6mg/ml respectively. Venom containing toxicity was analyzed by SDS-PAGE and revealed their protein bands with an apparent molecular weight of 25 kDa, 41 kDa and 95 kDa for <i style="mso-bidi-font-style:normal">T. javana </i>and 28 kDa and 66 kDa for <i style="mso-bidi-font-style:normal">L. indica</i>. Many components with molecular mass ranging 29-97kDa exhibited prominent caseinolytic and gelatinolytic activities with prominent hemolytic acitivty upto the dilution of 10^-6. The lethal concentration, LC₅₀ was determined in <i style="mso-bidi-font-style:normal">Artemia salina</i>. In addition, the quantitative status of free amino acids was performed through HPTLC. Presence of peptides was revealed by using HPLC, which was indicative of viable biological properties.