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Title: Isolation of aliphatic-antibiotic compounds from marine invertebrate, Heteractis magnifica ʽQuoy & Gaimard, 1833ʼ against captive marine ornamental fish pathogens
Authors: Gunasundari, V
Kumar, T T Ajith
Kumaresan, S
Balagurunathan, R
Balasubramanian, T
Keywords: Heteractis magnifica;Artemia nauplii;Hemolytic activity;Fish pathogen;Antimicrobial activity
Issue Date: Oct-2013
Publisher: NISCAIR-CSIR, India
Abstract: Present study is aimed to screen and isolate the antibiotic compounds from the mucus of Sea-anemone Heteractis magnifica. Mucus was extracted and characterized using gel chromatography, Reverse Phase High Performance Liquid Chromatography and spectroscopic methods. A molecular weight of purified protein was found to be 17KDa on SDS-PAGE. Protein content of mucus recorded was 0.62mg/ml - Mucus extract exhibited significant hemolytic activity in human, sheep, chicken and fish erythrocytes. Median lethal concentration was determined using Artemia nauplii. Antimicrobial activity screening was performed against the ten isolated bacterial pathogens and well inhibits the growth of Aeromonas hydrophila, Flavobacterium sp., Pseudomonas fluorescens, Micrococcus sp., and Streptococcus sp. The aliphatic nature of the mucus was determined by using FTIR spectroscopic analysis. This is the first report on the aliphatic-antibiotic compounds derived from Heteractis magnifica mucus which is used as marine ornamental fish disease controlling agent.
Page(s): 807-811
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.42(6) [October 2013]

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