Formulation of medium constituents by multiresponse analysis of central composite design to enhance chitinase production in <i>Pantoea dispersa</i>

Abstract

In the present study, a high chitinase producing strain <i>Pantoea dispersa </i>was isolated from the sea dumps at Bhavnagar, India. Chitin, urea, CaCl₂ and MgSO₄.7H₂O were variables used in central composite design for chitinase production. Chitinase, biomass and <i>p</i>H were the responses used in different models to evaluate individually fit ones. Quadratic model

was found to be fit for chitinase response whereas in the case of biomass and <i>p</i>H, linear model was found to be fit without the effect of others. Chitinase production was optimized with respect to other responses such as biomass and <i>p</i>H in multiresponse analysis of response surface design by using desirability approach. In multiresponse analysis, following medium formulation (g/l), chitin, 15; urea, 0.32; CaCl₂, 0.10 and MgSO₄.7H₂O, 0.08 was found to predict optimum chitinase production of 482.77 units/ml with overall highest desirability of 0.854 as compared to other formulation s. The selection of model was done on the basis of hi gh Adjusted R-squared value and lowered <i>p</i>-value for each model in individual analysis of

each response. In multiresponse experiment, it was found that for response chitinase quadratic model and for responses <i>p</i>H and biomass linear models were well fit. Through desirability analysis, it was found that in the chitinase production, <i>p</i>H was essential as compared to biomass in <i>P. dispersa. </i>Endochitinase and chitobiase actvities were also studied.