Please use this identifier to cite or link to this item: http://nopr.niscpr.res.in/handle/123456789/23392
Title: Significance of regional difference in ion concentrations in lizard, Hemidactylus flaviviridis (Ruppell): Assessment of ionic influence on sperm motility in vitro
Authors: Rai, Umesh
Nirmal, B K
Keywords: Hemidactylus flaviviridis;Ion concentration;Ionic influence;Lizard;Sperm mobility
Issue Date: Dec-2003
Publisher: NISCAIR-CSIR, India
Abstract: A gradual increase in the concentration of Ca2+ from anterior to the posterior region was observed when mono- and divalent cations were estimated in different segments of the epididymis in wall lizard. Na+ and K+ levels increased from anterior to middle segment but declined significantly in the posterior segment. However, no significant difference in the levels of Mg2+ was observed in various segments. To study the influence of mono- and divalent cations on sperm motility in vitro, the spermatozoa from posterior region of the epididymis were incubated in medium with varying concentrations of Na+ K+, Ca2+ and  Mg2+. Spermatozoa were non-motile when suspended in Na+-free medium. Addition of  NaCI induced the acquisition of sperm motility in a concentration-dependent manner. Further, amiloride, a Na+-influx blocker, markedly reduced the Na+-induced forward progressive motility. Unlike Na+, the presence of K+ or Ca2+in the incubation   medium reduced the motility of spennatozoa even at very low concentrations. The inhibitory effect of Ca2+ decreased when nifedipine, a Ca2+-influx blocker, was added to the medium. Mg2+ at high concentrations only was able to reduce the forward progressive motility.
Page(s): 1431-1435
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.41(12) [December 2003]

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