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http://nopr.niscpr.res.in/handle/123456789/21935| Title: | Covalent labelling at 5'-end of synthetic oligonucleotides by one step generation of alkylamino group |
| Authors: | Dubey, Krishna K Singh, Yashveer Kumar, Sanjay Singh, Ramendra K Misra, Krishna |
| Issue Date: | Jun-2002 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Labelling of oligonucleotides can be carried out at 5'- and / or 3'-positions, phosphate backbone or nucleobases. The widely used labelling strategies, however, involve labelling of 5'- position where the label is covalently attached through a linker. Usually -NH2 or -SH functionalities are generated for coupling of the reporter groups. In the present work, a rapid one step procedure for generating 5'-alkylamino function has been developed.5'-Butylamino thymidine and 5'-butylaminod (TGCT) have been synthesised using solid phase synthesis. The efficacy of the method reported herein has been evaluated by labelling 5'- position with a fluorophore, 4-acetylamino-1, 8-naphthalimido-N-caproic acid (NCA). |
| Page(s): | 1246-1250 |
| ISSN: | 0975-0983(Online); 0376-4699(Print) |
| Appears in Collections: | IJC-B Vol.41B(06) [June 2002] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJCB 41B(6) 1246-1250.pdf | 1.03 MB | Adobe PDF | View/Open |
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