Antiepileptic potential of <i style="mso-bidi-font-style:normal">Anisomeles indica </i>(Linn.)<i style="mso-bidi-font-style:normal"> </i>Kuntze aerial parts in pentylenetetrazole-induced experimental convulsions in Wistar rats

Abstract

The chloroform (4.20% w/w), ethyl acetate (4.23% w/w) and aqueous decoction (12.11% w/w) extracts of the aerial parts of <i style="mso-bidi-font-style:normal">A. indica</i> were screened for the antiepileptic activity against maximal electroshock (MES) model and pentylenetetrazole (PTZ) models at&nbsp; doses of 200, 400 mg/kg, po once. Phenytoin and diazepam (25 and 2 mg/kg,<i style="mso-bidi-font-style:normal"> </i>ip) were used as standard drugs in MES and PTZ model, respectively. Further, ethyl acetate extract (active extract) was fractionated into flavonoid and tannin fraction, which were subsequently evaluated for the antiepileptic potential against both MES and PTZ models at a dose of 50 mg/kg, po<i style="mso-bidi-font-style:normal">. </i>Pretreatment with ethyl acetate extract 200, 400 mg/kg, po, for 1 week showed significant antiepileptic activity against PTZ induced convulsions only. Isolated flavonoid fraction showed more potent antiepileptic activity as compared to ethyl acetate extract, without any neurotoxic effect. However, tannin fraction did not produce antiepileptic activity against PTZ induced convulsions. It may be concluded that the flavonoids fraction of ethyl acetate extract of aerial parts of <i style="mso-bidi-font-style:normal">A. indica,</i> but not the aqueous decoction has antiepileptic potential, without producing neurotoxic effects.