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|Title:||Purification and characterization of Inulinase from marine bacterium, Bacillus cereus MU-31|
|Keywords:||Bacillus cereus;Extracellular inulinase;Fructose;Inulin|
|Abstract:||Marine bacterium MU-31 strain isolated from sediment of Vellar estuary Parangipettai (Southeast coast of India) was found to secrete a large amount of inulinase into the medium. It was identified as this marine bacterium potent isolated strain was identified as Bacillus cereus by various biochemical studies. Optimum reaction conditions were as follows: temperature, 30°C; pH, 7.0; NaCl, 2%; substrate concentration, 1.5%; carbon sources, inulin and nitrogen sources, yeast extract. Purified enzyme gave a single band on gel electrophoresis and its molecular weight was estimated to be 66 kDa. Among the different chemicals used, only SDS enhanced the enzyme production and 1,10-phenanthroline was strongly inhibited the inulinase production. Among metal ions, Mg2+ stimulated inulinase activity and strongly inhibited by Hg2+ and Pb2+ at 1mM concentration. All these conditions make Bacillus cereus MU-31 a potential candidate for industrial enzymatic production of high fructose syrup and in other large-scale biotechnological processes.|
|ISSN:||0975-1033 (Online); 0379-5136 (Print)|
|Appears in Collections:||IJMS Vol.42(4) [August 2013]|
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