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http://nopr.niscpr.res.in/handle/123456789/19867| Title: | Mass Cultivation of Bacillus thuringiensis var. kurstaki in Fed-Batch Culture for High Spore Count and Improved Insecticidal Activity |
| Authors: | Bihari, Vinod Tripathi, C K M Sur, Banani Liu, W M Bajpai, Rakesh |
| Keywords: | Insecticidal activity;Fed-batch fermentation;Sporulation;Crystal potency;Glucose feeding |
| Issue Date: | Apr-2002 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Batch and fed-batch cultivation of Bacillus thuringiensis var. kurstaki was carried out to achieve high cell and spore density with maximum insecticidal activity. Cell cultivation was done in fully equipped fermentors under controlled conditions at different feed rates. Increased cell mass, high spore counts and improved insecticidal activity were achieved with fed-batch cultivation system. With the appropriate supply of glucose and other nutrients, it was possible to grow the cells to >50 g (dry weight)/l density and achieve a spore concentration of 3.9×1010/ml having high insecticidal activity. |
| Page(s): | 205-208 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.01(2) [April 2002] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 1(2) 205-208.pdf | 898.56 kB | Adobe PDF | View/Open |
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