Please use this identifier to cite or link to this item: http://nopr.niscpr.res.in/handle/123456789/1939
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dc.contributor.authorGao, Z Q-
dc.contributor.authorMeng, C X-
dc.contributor.authorWang, G C-
dc.contributor.authorYe, N H-
dc.date.accessioned2008-09-01T10:51:15Z-
dc.date.available2008-09-01T10:51:15Z-
dc.date.issued2008-08-
dc.identifier.issn0301-1208-
dc.identifier.urihttp://hdl.handle.net/123456789/1939-
dc.description244-249en_US
dc.description.abstractThylakoid membranes were isolated and purified from diploid filamentous sporophytes of Porphyra yezoensis Ueda using sucrose density gradient ultracentrifugation (SDGUC). After thylakoid membranes were solubilized with SDS, the phtosystem II (PSII) particles with high 2, 6-dichloroindophenol (DCIP) photoreduction activity were isolated by SDGUC. The absorption and fluorescence spectra, DCIP photoreduction activity and oxygen evolution activity of the thylakoid membranes and PSII particles were determined. The polypeptide composition of purified PSII particles was distinguished by SDS-PAGE. Results showed that PSII particles of sporophytes differed from the gametophytes in spectral properties and polypeptide composition. Apart from 55 kDa D1-D2 heterodimer, CP47, CP43, 33 kDa protein, D1, D2, cyt b559 and 12 kDa protein were identified from PSII particles from sporophytes; a new 102 kDa protein was also detected. However, cyt c-550, 20 kDa, 14 kDa and 16 kDa proteins found in PSII particles from gametophytes were not detected in the sporophytes.en_US
dc.language.isoen_USen_US
dc.publisherCSIRen_US
dc.sourceIJBB Vol.45(4) [August 2008]en_US
dc.subjectPorphyra yezoensisen_US
dc.subjectPhotosystem IIen_US
dc.subjectFilamentous sporophytesen_US
dc.subjectThylakoid membranesen_US
dc.subjectPolypeptide compositionen_US
dc.subjectSucrose density gradient ultracentrifugationen_US
dc.titleIsolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensisen_US
dc.typeArticleen_US
Appears in Collections: IJBB Vol.45(4) [August 2008]

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