Naringenin application alters function and metabolism of pea nodules

Abstract

<span style="font-size:14.0pt;line-height: 115%;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" color:black;mso-ansi-language:en-in;mso-fareast-language:en-in;mso-bidi-language:="" hi"="" lang="EN-IN">Application of naringenin at the time of sowing influenced metabolism of pea (<i>Pisum sativum</i>)<i> </i>nodules at various stages of plant growth. Proportion of assimilated ¹⁴C was higher in nodules of plants from naringenin treatment as compared to control plants. Activities of enzymes of ammonia assimilation, viz. glutamine synthetase (GS), glutamate synthase (GOGAT), glutamate dehydrogenase and carbohydrate metabolism, viz. phosphoenolpyruvate carboxylase (PEPCase), malate dehydrogenase (MDH), isocitrate dehydrogenase and enzymes of Entner Dudoroff pathway were enhanced by naringenin treatment. Naringenin also increased the acetylene reduction activity of nodules, which positively correlated with increase in GS, GOGAT, PEPCase and MDH activities. However, there was no effect of naringenin application on key enzymes of hexose monophosphate (HMP) pathway, viz. glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase.</span>