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dc.contributor.authorPrashanth, T-
dc.contributor.authorReddy, G R-
dc.contributor.authorSuryanaryana, V V S-
dc.contributor.authorDechamma, H J-
dc.identifier.issn0975-1009 (Online); 0019-5189 (Print)-
dc.description.abstractDendritic cells (DC) which are located at the interface of innate and adaptive immunity are targets of infection by many RNA and DNA viruses. Advances in the ex vivo generation of monocyte derived non proliferating dendritic cells have been used for clinical application like immunotherapy. IL-4 cytokine plays essential role in the maturation and generation of DCs. Bos indicus interleukin 4 (boIL-4) 408 bp was amplified from PBMC’s and cloned in pBSIIKS+ vector. The sequence analysis showed N terminal 69 bp signal sequence and one N-glycosylation site. The phylogenetic tree analysis showed that Bos indicus IL-4 is closely related to the ruminant IL-4 and least sharing of genetic line of human and mouse IL-4. The recombinant boIL-4 protein was expressed in CHO cells which secreted a 16 kDa protein which was confirmed by SDS PAGE and western blotting. The rec-boIL-4 protein proliferated the bovine PBMC’s, decreased production of nitric oxide in antigen stimulated macrophages, and phagocytosed the  micro particles confirming its activity on dendritic cells.en_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJEB Vol.51(05) [May 2013]en_US
dc.subjectChinese hamster ovary cellsen_US
dc.subjectDendritic cellsen_US
dc.subjectNitric oxideen_US
dc.titleCloning and expression of Bos indicus interleukin-4 in mammalian cellsen_US
Appears in Collections:IJEB Vol.51(05) [May 2013]

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