Please use this identifier to cite or link to this item:
Title: In vitro propagation of Dendrobium macrostachyum Lindl.—A threatened orchid
Authors: Pyati, A N
Murthy, H N
Hahn, E J
Paek, K Y
Issue Date: May-2002
Publisher: NISCAIR-CSIR, India
Abstract: In vitro propagation of Dendrobium macrostachyum, a threatened and endemic species was achieved through nodal explants. The nodal explants were cultured on Murashige and Skoog (MS) basal medium and MS medium supplemented with N6-benzyladenine (BA-2.22, 4.44 and 8.88 μM ), Kinetin (KN - 2.32, 4.65, and 9.29 μM) and Coconut water (CW, 5, 10 and15%) individually or in combination with 2.69 μM α- naphthalene acetic acid (NAA). Axillary shoots were induced directly from nodal explants in medium containing BA, KN or CW. Optimal shoot induction (6 shoots/explant) was attained from nodal exp lants cultured on medium supplemented with 15% CW. Well developed shoots rooted at an average 5 roots per shoot in half strength MS medium devoid of any growth regulators.
Page(s): 620-623
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.40(05) [May 2002]

Files in This Item:
File Description SizeFormat 
IJEB 40(5) 620-623.pdf753.17 kBAdobe PDFView/Open

Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.