<i><span style="font-size:14.0pt;line-height: 115%;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:black;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">In vitro </span></i><span style="font-size:14.0pt;line-height: 115%;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:black;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">propagation of <i>Dendrobium macrostachyum </i>Lindl.—A threatened orchid</span>

Abstract

<i><span style="font-size:14.0pt;line-height: 115%;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" color:black;mso-ansi-language:en-in;mso-fareast-language:en-in;mso-bidi-language:="" hi"="" lang="EN-IN">In vitro </span></i><span style="font-size:14.0pt;line-height: 115%;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" color:black;mso-ansi-language:en-in;mso-fareast-language:en-in;mso-bidi-language:="" hi"="" lang="EN-IN">propagation of <i>Dendrobium macrostachyum, </i>a threatened and endemic species was achieved through nodal explants. The nodal explants were cultured on Murashige and Skoog (MS) basal medium and MS medium supplemented with N⁶-benzyladenine (BA-2.22, 4.44 and 8.88 μM ), Kinetin (KN - 2.32, 4.65, and 9.29 μM) and Coconut water (CW, 5, 10 and15%) individually or in combination with 2.69 μM α- naphthalene acetic acid (NAA). Axillary shoots were induced directly from nodal explants in medium containing BA, KN or CW. Optimal shoot induction (6 shoots/explant) was attained from nodal exp lants cultured on medium supplemented with 15% CW. Well developed shoots rooted at an average 5<i> </i>roots per shoot in half strength MS medium devoid of any growth regulators.</span>