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Title: In vitro control of fasciation in proliferating nucel1ar embryos of Mangifera indica L.var totapari red small for cloning
Authors: Chaturvedi, H C
Agnihotri, S
Sharllla, M
Sharma, A K
Jain, M
Chourasia, A
Keywords: Convertibility of embryos;Dwarfing rootstock;Ethylene inhibitors;Fasciation of embryos;Mangifera indica totaparired small;Nucellar embryos
Issue Date: Nov-2003
Publisher: NISCAIR-CSIR, India
Abstract: Nucellar tissue contained in ovular halves of young fruits of Mangifera indica L. totapari red small, a dwarfing rootstock, differentiated fasciated embryonal structures in presence of 6-benzylaminopurine [BA P(0.15 mg l-1)] 1, 6-(γ-γ-dimethylallylamino) purine [2iP(0.15 mg l-1)] and indole-3- acetic acid [(IAA(0.5 mg l-1)] incorporated in the semisolid medium during 50-60 days. Due to embryonal fasciation, hardly 2-3 well-formed embryos could be obtained per culture of proliferating embryos. Of the 3 ethylene inhibitors [ L-α-(2-aminoethoxyvinyl)-glycine-HCl (AVG), AgNO3, and salicylic acid (SA)]used, embryonal fasciation and necrosis of intervening tissue was completely control led by 3-4 subcultures of fasciated mass of embryos under the influenee of AVG (0.05 mg l‑1) in presence of adenine sulphate [AdS (50mg l-1)] incorporated in the same medium. Almost synchronized development of isolated embryos, measuring ca 2 cm in length, was observed in a different medium used in liquid stationary state and supplemented. particularly with stress-producing substances [abscisic acid (ABA 0.01 mgl-1) : and polyethylene glycol (PEG, 100 mg l-1) besides certain other modifications, About 34% convertibility of processed embryos was obtained during a period of 90 days. The plantlets had well -developed roots along with laterals which were longer than leafy shoots. In vitro raised plants survived ex vitro for about 2 months.
Page(s): 1311-1316
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.41(11) [November 2003]

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