<span style="font-size:14.0pt;line-height: 115%;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#252525;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">Influence of <span style="font-size:14.0pt;line-height: 115%;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#0F0F0F;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">nutritional <span style="font-size:14.0pt;line-height: 115%;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#3B3B3B;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">supp<span style="font-size:14.0pt;line-height:115%; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#0F0F0F;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">lements <span style="font-size:14.0pt;line-height:115%; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#252525;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">on keratinolysis by <i>Am</i><i><span style="font-size: 14.0pt;line-height:115%;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";color:#4C4C4C;mso-ansi-language:EN-IN;mso-fareast-language: EN-IN;mso-bidi-language:HI" lang="EN-IN">yc<span style="font-size:14.0pt; line-height:115%;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; color:#252525;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language: HI" lang="EN-IN">olatopsis keratinophila</span></span></i></span></span></span></span></span>

Abstract

Keratinolytic potential of A. <i>keratinophila</i> (DSM 44409^T) , a newly described <i>Amycolatopsis</i> sp. isolated from cultivated soil in Kuwait, was demonstrated using keratinazure as the sole source of carbon and nitrogen as estimated by gel diffusion assay . Effects of 12 various nutritional supplements on the keratinolytic and azocollytic activities were determined .NH₄H₂PO₄and KNO₃ in the medium supported a significantly higher keratinolytic activity than other supplements. However, azocollytic activities in all the supplemented media and the control were same. Best combination of carbon and nitrogen supplements (galactose and NH₄H₂PO₄respectively) used to evaluate the dynamics of growth and enzymes (keratinase and protease) activities of the isolate revealed a luxuriant growth with optimal keratinolytic activity occurring during the log phase. Other parameters of the fermentation medium, including <i>p</i>H, biomass accumulation, total protein and free amino acid concentrations were also studied.