Modulatory effect of diclofenac on antispasmodic effect of pitofenone in cholinergic spasm

Abstract

<span style="font-size:12.0pt;line-height: 115%;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" mso-ansi-language:en-in;mso-fareast-language:en-in;mso-bidi-language:hi"="" lang="EN-IN">Biliary, ureteric and intestinal colic are extremely common clinical conditions associated with smooth muscle spasm. In the present study, antispasmodic activity was carried out against acetylcholine (10-640 ng/ml)-induced contractions on guinea pig ileum. Acetylcholine (10-640 ng/ml) induced concentration-dependent contraction of smooth muscle. Diclofenac, in varying concentration (9.4 x 10^-5 mol/l and 14.1 x 10^-5 mol/l) shifted the concentration response curve of acetylcholine to the right without sppressing the maximal response. However, in higher concentration diclofenac (18.9 x 10^-5 mol/l) blocked the response in an unsurmountable fashion. Further, analgin (11.09 x 10^-5 , 16.63 x 10^-5 and 22. 18 x 10^-5 mol/l) in equimolar concentrations did not alter the concentration response curve of acetylcholine, but in higher concentration analgin (44.36 x10^-5 mol/l) also blocked the response in an unsurmountable fashion. Pitofenone (2 .5 x 10^-6mol/l) also, shifted the concentration response curve of acetylcholine to right in a parallel fashion with no change in maximal response. The present study confirms the potent antispasmodic activity of diclofenac-pitofenone combination in comparison to analgin –pitofcnone in molar equivalcnt concentration (in comparison to diclofenac) against acetylcholine-induced contractions of guinea pig ileum.</span>