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Title: Plant growth promoting rhizobacteria mediated improvement of health status of tea plants
Authors: Chakraborty, U
Chakraborty, B N
Chakraborty, A P
Sunar, K
Dey, P L
Keywords: Bacillus amyloliquefaciens;B. pumilus;Camellia sinensis;Carrier based bioformulation;Growth promotion;Serratia marcescens
Issue Date: Jan-2013
Publisher: NISCAIR-CSIR, India
Abstract: Plant growth promoting rhizobacteria (PGPR) have immense potential application in sustainable agriculture as ecofriendly biofertilizers and biopesticides. The present study was undertaken to explore the potential of such microorganisms from the rhizosphere of tea [Camellia sinensis (L.) O. Kuntze] for the overall improvement in growth and productivity of tea, which is the most important crop of this region. Isolation and testing of bacteria for PGPR activities revealed that a large number of them showed such activities. Of which three were selected for various studies. The selected bacteria were Bacillus amyloliquefaciens, Serratia marcescens and B. pumilus. These bacteria showed positive PGPR traits in vitro, such as, phosphate solubilization, siderophore production, antagonism to pathogens and IAA production. 16S rDNA sequencing of the bacteria was done and their phylogenetic relationships determined. Under in vivo conditions, the PGPR enhanced the seedling growth of tea varieties in the nursery as well as in the field. Plant growth promotion was determined in terms of increase in number of leaves, their biomass and number of shoots. In order to determine the tolerance of bacteria to insecticides, in vitro tests were conducted, which indicated that PGPR could tolerate more than 100 times the concentration applied in the field. Sustainability of the applied bacteria in soil was tested by PTA-ELISA and Dot immunobinding assay using polyclonal antibodies raised against the PGPR. Certain bioformulations of the PGPR in talc powder, saw dust and rice husk also been prepared and their viability tested. The bacteria showed good survivability even up to 9 months of storage. Application of the PGPR led to enhancement in activities of defense related enzymes, such as, phenyl alanine ammonia lyase, peroxidase, chitinase and β-1,3-glucanase, in tea leaves. Total phenols also increased quantitatively. It is evident from the present study that application of PGPR in the soil lead to biopriming of the plants through induced systemic resistance and other mechanisms.
Page(s): 20-31
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.12(1) [January 2013]

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