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|Title:||Ribosome-inactivating property of gelonin is more affected by N-succinimidyl 6-[3-(2-pyridyldithio) propionamido]hexanoate modification than N-succinimidyl-3- (2-pyridylthio) propionate|
Singh, Ranjit C
Dubey, Rajesh Kumar
|Abstract:||As gelonin cross-linked to carrier proteins has been found as an effective hybrid complex for selective targeting to specific cells, the ε-NH2 group of gelonin(s) obtained by different methods has been sequentially modified by N-succinimidyl 6-[3-(2-pyridyldithio) propionamido] hexanoate (long chain-SPDP) and its effect on immunoreactivity and ribosome inactivating property has been compared with that of N-succinimidyl-3-(2-pyridylthio) propionate (SPDP) modified gelonin. Modification of single amino group results in about 80% inhibition of immunoreactivity and more than 90% loss of protein synthesis-inhibition activity. Modification of 2-3 amino groups further hampers both immunoreactivity and protein synthesis inhibition property of gelonin. Upon comparison of long chain-SPDP with SPDP modification, the long chain-SPDP modification plays more pronounced effect on immunoreactivity and ribosome-inactivating property (RIP) activity than that of SPDP. It may, therefore, be concluded that the increase in carbon-chain spacer arm, although may provide less steric hindrance for receptor recognition of the carrier protein, has inhibitory effect on the cytotoxic activity of gelonin .|
|ISSN:||0975-0959 (Online); 0301-1208 (Print)|
|Appears in Collections:||IJBB Vol.37(3) [June 2000]|
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