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dc.contributor.authorSingh, Vinod-
dc.contributor.authorSingh, Ranjit C-
dc.contributor.authorDubey, Rajesh Kumar-
dc.contributor.authorAlam, Anis-
dc.identifier.issn0975-0959 (Online); 0301-1208 (Print)-
dc.description.abstractAs gelonin cross-linked to carrier proteins has been found as an effective hybrid complex for selective targeting to specific cells, the ε-NH2 group of gelonin(s) obtained by different methods has been sequentially modified by N-succinimidyl 6-[3-(2-pyridyldithio) propionamido] hexanoate (long chain-SPDP) and its effect on immunoreactivity and ribosome inactivating property has been compared with that of N-succinimidyl-3-(2-pyridylthio) propionate (SPDP) modified gelonin. Modification of single amino group results in about 80% inhibition of immunoreactivity and more than 90% loss of protein synthesis-inhibition activity. Modification of 2-3 amino groups further hampers both immunoreactivity and protein synthesis inhibition property of gelonin. Upon comparison of long chain-SPDP with SPDP modification, the long chain-SPDP modification plays more pronounced effect on immunoreactivity and ribosome-inactivating property (RIP) activity than that of SPDP. It may, therefore, be concluded that the increase in carbon-chain spacer arm, although may provide less steric hindrance for receptor recognition of the carrier protein, has inhibitory effect on the cytotoxic activity of gelonin .en_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJBB Vol.37(3) [June 2000]en_US
dc.titleRibosome-inactivating property of gelonin is more affected by N-succinimidyl 6-[3-(2-pyridyldithio) propionamido]hexanoate modification than N-succinimidyl-3- (2-pyridylthio) propionateen_US
Appears in Collections:IJBB Vol.37(3) [June 2000]

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