A new membrane probing steroidal spin label: Synthesis and applications

Abstract

<span style="font-size:14.0pt;font-family:HiddenHorzOCR;mso-bidi-font-family:HiddenHorzOCR">The applicability of a new steroidal spin label, <span style="font-size:14.0pt;font-family:HiddenHorzOCR; mso-bidi-font-family:HiddenHorzOCR">3-oxo-androstan-17β<span style="font-size:14.0pt;font-family:HiddenHorzOCR;mso-bidi-font-family:HiddenHorzOCR">-yl-(2",2",6",6"-tetramethyl-N-oxyl) piperidyl butan- 1', 4'-dioate, in studying the phase transition properties of model membrane L-α-dipalmitoyl phosphatidyl choline (DPPC) in the presence and absence of drugs has been explored. Its synthesis and characterization has been described, herein. Besides, the localization of this spin label in lipid liposomes has been studied using electron spin resonance (ESR), differential scanning calorimetry (DSC) and ¹H and ³¹P NMR spectroscopic techniques. The label has also been used to study the permeability of <span style="font-size:14.0pt;font-family: HiddenHorzOCR;mso-bidi-font-family:HiddenHorzOCR">epinephrine into membrane. The results show that the spin label has a good potential as a spin

probe in the study of biomembranes.

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