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http://nopr.niscpr.res.in/handle/123456789/14632
Title: | Sequential cellulase production, saccharification and ethanol fermentation using rice straw |
Authors: | Pasha, Chand Sekhar, Banoth Chandra Srinivas, Banoth Balakrishna, Kannoju Hanumalal, Nunavath |
Keywords: | A. niger;Cellulases;Rice straw;Sequential saccharification and fermentation;S. cerevisiae (OC21) |
Issue Date: | Sep-2012 |
Publisher: | NISCAIR-CSIR, India |
Abstract: | This study presents alkali pretreated rice straw for cellulase production using Aspergillus niger CP1 by solid state fermentation (SSF). On 8th day, 140±2.4 IU g/l dry substrate (ds) carboxy methyl cellulose CMCase), 15±1.2 IU g/l ds filter paper activity (FPA) and 24±1.1 IU g/l ds â-glucosidase activities were noted. On extraction of 120 g material with 1 l of 0.1 mol/l citrate buffer, 5.9±0.12 IU ml/l CMCase, 0.62±0.001 IU ml/l FPA and 1.11±0.002 IU/ml â-glucosidase were obtained. When extracted enzyme was used for saccharification of 120 g fresh pretreated rice straw containing 100 g holocellulose, 45±0.2 g, sugars (50 g/l) were released in 900 ml hydrolysate to give 45% hydrolysis. Hydrolysate after yeast fermentation gave 15.6±0.05 g/l ethanol with a yield of 0.4±0.011 g/l/h and 78% fermentation efficiency. In sequential enzyme production, saccharification and fermentation studies, on 8thday of cellulase production by SSF, temperature was shifted from 28±1°C to 55°C for 36 h, and 76% hydrolysis was achieved. When A. niger in hydrolysate was inactivated and used, 68±0.83 g/l ethanol and 92% fermentation efficiency were obtained. Hydrolysate used without inactivation of A. niger gave 61.65±0.78 g/l ethanol and 85% fermentation efficiency. |
Page(s): | 616-620 |
ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
Appears in Collections: | JSIR Vol.71(09) [September 2012] |
Files in This Item:
File | Description | Size | Format | |
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JSIR 71(9) 616-620.pdf | 61.72 kB | Adobe PDF | View/Open |
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